This guide provides a comprehensive protocol for planning a BioBlitz event in Eastern Ontario. It covers the purpose and goals of a BioBlitz, how to select a location, how to plan the event, how to collect data, and how to undertake species identification. The guide also includes information on who can help, where to start, and what to expect during the event. The goal of the guide is to simplify the planning process and encourage more events by providing a protocol and resources for planning a BioBlitz.